ISO/TS 5354-2:2024-分子生物标志物纺织品生产用棉花中DNA的检测第2部分:基于聚合酶链式反应（PCR）的棉花转基因事件检测方法的目标序列概述-国家标准馆·国家数字标准馆

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Molecular biomarkers — Detection of DNA in cotton used for textile production — Part 2: Overview of target sequences for use in polymerase chain reaction (PCR)-based detection methods for cotton genetically modified (GM) events 分子生物标志物纺织品生产用棉花中DNA的检测第2部分:基于聚合酶链式反应（PCR）的棉花转基因事件检测方法的目标序列概述

发布日期： 2024-04-19

                本文件提供了可用于筛选棉花和棉花产品中转基因(GM)材料存在的靶序列列表。
本文件适用于可从中提取足够高质量PCR扩增DNA的棉籽、棉叶、棉纤维和棉纤维衍生材料。
描述从不同棉花样品中提取DNA的方法可以在ISO？5354-1[1].
笔记？1？靶序列列表为筛选所有目前已知的转基因棉花事件和含有相同DNA序列的转基因棉花事件提供了指导。CEN/TS中提供了关于食品筛选的进一步指导？16707[2].
笔记？2？取样超出了本文档的范围。有关棉花产品抽样的信息可在ISO？1130:1975[3]和ASTM？D1441-12[4]。

This document provides a list of target sequences that can be used to screen for the presence of genetically modified (GM) material in cotton and cotton products.
This document is applicable to cottonseed, cotton leaf, cotton fibre and cotton fibre-derived materials from which sufficiently high-quality PCR amplifiable DNA can be extracted.
Methods describing the extraction of DNA from different cotton samples can be found in ISO 5354-1[1].
NOTE 1        The list of target sequences provides guidance for the screening of all currently known GM cotton events and GM cotton events that contain the same DNA sequences. Further guidance on screening of foodstuffs is provided in CEN/TS 16707[2].
NOTE 2        Sampling is outside of the scope of this document. Information on sampling cotton products can be found in ISO 1130:1975[3] and in ASTM D1441-12[4].