1.1 这种环境室方法测量处理过的湿蓝色和湿白色在四周的时间内对孢子萌发和随后的营养生长的抗性。该测试方法可用于估计杀菌剂的性能，并有助于预测真菌生长开始前湿蓝和湿白的储存时间。该装置的设计使得它可以由任何感兴趣的一方容易地建造或获得，并复制湿蓝和湿白接种真菌孢子的自然环境。在未经处理或处理的湿蓝色和湿白色上萌发的孢子可产生真菌生长，导致湿蓝色和湿白色的毁容或变色，或两者兼而有之。 1.2 以国际单位制单位表示的数值应被视为标准。本标准不包括其他计量单位。 1.3 本标准无意解决与其使用相关的所有安全问题（如果有的话）。本标准的使用者有责任在使用前建立适当的安全、健康和环境实践，并确定法规限制的适用性。 1.4 本国际标准是根据世界贸易组织技术性贸易壁垒委员会发布的《关于制定国际标准、指南和建议的原则的决定》中确立的国际公认的标准化原则制定的。 = 意义和用途 ===== 6.1 环境室法是一种加速试验，用于确定湿蓝和湿白对真菌生长的抵抗力，真菌是霉菌的病原体。参见测试方法 D3273 . , 6.2 环境室法可用于估计杀菌剂的性能，并有助于预测真菌生长开始前的储存时间。 6.3 环境室法复制了自然环境，其中湿蓝色或湿白色接种了真菌孢子，随后被真菌变形或变色。 6.4 环境室法测量处理过的湿蓝色或湿白色对孢子萌发和随后的营养生长的抗性，该营养生长在四周的时间内传播在相对较大的湿蓝色或湿白色样品的表面上。 6.5 通过添加来自当前运行的制革厂的具有真菌生长的湿蓝色和湿白色样品，可以保持环境室接种代表制革厂中发现的真菌的真菌。6.6 未经杀真菌剂处理的湿蓝色和湿白色的对照样品可以定期添加到腔室中，以增加腔室中的真菌生长水平。 6.7 将杀菌剂从测试样品浸出到琼脂中通常会导致培养皿测试中的真菌生长抑制区，但在环境室中，任何从测试样品浸出的杀菌剂都会滴入室中包含的水中，因此不会导致培养皿测试中观察到的错误读数类型。

1.1 This environmental chamber method measures the resistance of the treated Wet Blue and Wet White to the germination of spores and subsequent vegetative growth over a period of four weeks. The test method is useful in estimating the performance of fungicides and should assist in the prediction of storage time of Wet Blue and Wet White before fungal growth begins. The apparatus is designed so it can be easily built or obtained by any interested party and duplicate the natural environment in which Wet Blue and Wet White is inoculated with fungal spores. Spores that germinate on untreated or treated Wet Blue and Wet White can produce fungal growth, resulting in disfigurement or discoloration, or both, of the Wet Blue and Wet White. 1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. ====== Significance And Use ====== 6.1 The environmental chamber method is an accelerated test for determining the resistance of Wet Blue and Wet White to the growth of fungi, the causal agent of mold. See Test Method D3273 . , 6.2 The environmental chamber method is useful in estimating the performance of fungicides and should assist in the prediction of storage time before fungal growth begins. 6.3 The environmental chamber method duplicates the natural environment in which Wet Blue or Wet White is inoculated with fungal spores and subsequently disfigured or discolored by fungi. 6.4 The environmental chamber method measures the resistance of the treated Wet Blue or Wet White to the germination of spores and subsequent vegetative growth that spreads over the surface of a comparatively large Wet Blue or Wet White specimen over a period of four weeks. 6.5 The environmental chamber can be kept inoculated with fungi representative of those found in tanneries by adding samples of Wet Blue and Wet White with fungal growth from currently operating tanneries. 6.6 Control specimens of Wet Blue and Wet White without fungicide treatment can be added to the chamber periodically to increase levels of fungal growth in the chamber. 6.7 Leaching of fungicide from the test specimen into the agar often causes a zone of inhibition of fungal growth in the Petri dish test, but in the environmental chamber any leaching of fungicide from the test specimen drips into the water contained in the chamber and thus does not cause the types of false readings observed in the Petri dish test.